Title | Metasurface-enhanced infrared spectroscopy in multiwell format for real-time assaying of live cells. |
Publication Type | Journal Article |
Year of Publication | 2023 |
Authors | Huang SH, Sartorello G, Shen P-T, Xu C, Elemento O, Shvets G |
Journal | Lab Chip |
Volume | 23 |
Issue | 9 |
Pagination | 2228-2240 |
Date Published | 2023 May 02 |
ISSN | 1473-0189 |
Keywords | Cell Culture Techniques, Cell Survival, Spectrophotometry, Infrared, Spectroscopy, Fourier Transform Infrared |
Abstract | Fourier transform infrared (FTIR) spectroscopy is a popular technique for the analysis of biological samples, yet its application in characterizing live cells is limited due to the strong attenuation of mid-IR light in water. Special thin flow cells and attenuated total reflection (ATR) FTIR spectroscopy have been used to mitigate this problem, but these techniques are difficult to integrate into a standard cell culture workflow. In this work, we demonstrate that the use of a plasmonic metasurface fabricated on planar substrates and the probing of cellular IR spectra through metasurface-enhanced infrared spectroscopy (MEIRS) can be an effective technique to characterize the IR spectra of live cells in a high-throughput manner. Cells are cultured on metasurfaces integrated with multiwell cell culture chambers and are probed from the bottom using an inverted FTIR micro-spectrometer. To demonstrate the use of MEIRS as a cellular assay, cellular adhesion on metasurfaces with different surface coatings and cellular response to the activation of the protease-activated receptor (PAR) signaling pathway were characterized through the changes in cellular IR spectra. |
DOI | 10.1039/d3lc00017f |
Alternate Journal | Lab Chip |
PubMed ID | 37010356 |
PubMed Central ID | PMC10159923 |
Grant List | R21 CA251052 / CA / NCI NIH HHS / United States R21 GM138947 / GM / NIGMS NIH HHS / United States UL1 TR002384 / TR / NCATS NIH HHS / United States UG3 CA244697 / CA / NCI NIH HHS / United States R01 CA194547 / CA / NCI NIH HHS / United States P01 CA214274 / CA / NCI NIH HHS / United States |